Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 3, Pages 285-293Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2009.11.045
Keywords
Acid hydrolysis/chymotrypsin; Acid hydrolysis/trypsin; Synechocystis sp PCC 6803; Trans-membrane domain; Integral membrane protein; HPLC; nano-LC-MS
Funding
- Korea Basic Science Institute K-MeP [T28021]
- Carl Trygger and Magnus Bergvall Foundation
- Chonbuk National University
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The identification of membrane proteins is currently under-represented since the trans-membrane domains of membrane proteins have a hydrophobic property. Membrane proteins have mainly been analyzed by cleaving and identifying exposed hydrophilic domains. We developed the membrane proteomics method for targeting integral membrane proteins by the following sequential process: in-solution acid hydrolysis, reverse phase chromatographic separation, trypsin or chymotrypsin digestion and nano-liquid chromatography-Fourier transform mass spectrometry. When we employed total membrane proteins of Synechocystis sp. PCC 6803, 155 integral membrane proteins out of a predictable 706 were identified in a single application, corresponding to 22% of a genome. The combined methods of acid hydrolysis-trypsin (AT) and acid hydrolysis-chymotrypsin (AC) identified both hydrophilic and hydrophobic domains of integral membrane proteins, respectively. The systematic approach revealed a more concrete data in mapping the repertoire of cyanobacterial membrane and membrane-linked proteome. (C) 2009 Elsevier B.V. All rights reserved.
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