Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 1, Pages 179-182Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.11.047
Keywords
Capillary IEF; MALDI-MS; MS; Syringe pump
Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R03AI099740] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R21EB010191] Funding Source: NIH RePORTER
- NIAID NIH HHS [R03 AI099740] Funding Source: Medline
- NIBIB NIH HHS [R21 EB010191, R21 EB010191-01A1] Funding Source: Medline
Ask authors/readers for more resources
This work presents several critical details for making cIEF-MALDI-MS a robust technique which will allow for more routine application and aid in automation. This includes emphasis on the hardware necessary for syringe pump mobilization and proper protocol for preventing disruption from gas bubbles. Following these guidelines, excellent elution time reproducibility is demonstrated for six pl markets (RSD < 5%). Additionally, the pl markets are used to calibrate the pH gradient and determine experimental pis of proteins detected offline by mass spectrometry. This was demonstrated using a standard protein mixture of myoglobin and two forms of beta-lactoglobulin. Experimental determination of protein pls and molecular weights were found to be in agreement with literature values. The technical details discussed provide a sound foundation for applying the offline coupling of MALDI-MS with cIEF. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available