Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 33, Pages 5420-5426Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.06.044
Keywords
beta-Lactam antibiotics; Molecularly imprinted polymer; Pseudo template; Photopolymerization; Molecularly imprinted solid-phase extraction
Funding
- National Natural Science Foundation of China [20805057, 20877091, 20890112]
- National Basic Research Program of China [2010CB933502, 2007CB407305]
- National High Technology Research and Development Program of China [2007AA06A407]
- National Water Project [2008ZX07207-002]
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To assess the potential risks associated with the environmental exposure of beta-lactam antibiotics (BLAs), the monitoring of the occurrence, distribution, and fate of these emerging contaminants in the environment is required. Herein, we demonstrate a molecularly imprinted solid-phase extraction (MISPE) method for selective and reliable screening of trace BLAs in river and tap water. By developing a low-temperature photopolymerization, highly selective molecularly imprinted polymers (MIPs) for five BLAs (penicillin G, amoxicillin, ampicillin, nafcillin and mezlocillin) were synthesized. Nafcillin was chosen as a pseudo template to make the MIP sorbent (Nafc-MIP), which was used in pseudo-template MISPE for preconcentration of the other four BLAs from river and tap water. The application of pseudo-template MISPE overcomes the template bleeding, which significantly elevates the sample background and restricts the application of MIP for detection of the target BLA below 2 mu g/L. The average recoveries of BLAs are in the range of 60-90% when Nafc-MIP was adopted as the selective MISPE sorbent. The developed method was validated, and applied to the screening of trace beta-lactam antibiotics in river and tap water. The linearity of the calibration curve for each BLA was observed over the range of 0.1-20 mu g/L (r > 0.998). The beta-lactam antibiotics were found within the range of 0-9.56 mu g/L in river water at the downstream of antibiotics manufacturers, and none were detected in the tap water. (C) 2010 Elsevier B.V. All rights reserved.
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