Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 11, Pages 2079-2086Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.10.082
Keywords
Aconitum carmichaelii; Diterpene alkaloids; Lipo-alkaloids; 14-Benzoylaconine-8-palmitate; 14-Benzoylaconine-8-linoleate; COX-1; COX-2; LTB4 formation; LD50; LC-APCI-MSn
Funding
- Hungarian State Eotvos Fellowship
- Aktion Osterreich-Ungarn and ARGE Alpen-Adria
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By optimizing the extraction and analytical conditions. a reliable and precise HPLC method coupled with photodiode array detection (HPLC-DAD) has been developed for the identification and quantification of three major aconitine-type alkaloids (aconitine, mesaconitine, hypaconitine) in the roots of Aconitum carmichaelii Debeaux. The qualitative analysis or the plant material was carried out by LC-APCI-MSn. By means of this method, 26 lipo-alkaloids were also identified from the roots of A. carmichaelii. The effect of processing on aconitine-type alkaloids, lipo-alkaloids and pure aconitine was studied. As part of our investigation, two lipo-alkaloids, 14-benzoylaconine-8-palmitate and 14-benzoylaconine-8-linoleate were produced semisynthetically. The COX-1, COX-2 and LTB4 formation inhibitory activity of aconite root extracts and different types of diterpene alkaloids and the toxicity of lipo-alkaloids were also investigated. (C) 2008 Elsevier B.V. All rights reserved.
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