Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 48, Pages 8505-8511Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.10.006
Keywords
Countercurrent chromatography; Multiple dual-mode; Enantiomer separation; Chiral selector
Funding
- Ministerio de Ciencia a Innovacion
- European Regional Development Fund (ERDF) [CTQ 2006-03378/PPQ]
- Ministerio de Educacion of Spain
- HEIF4
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Countercurrent chromatography (CCC) is a liquid-liquid chromatographic technique without a solid support. Several alternative elution modes can be applied to take advantage of the special nature of the liquid stationary phase. Among these dual-mode (DM) and multiple dual-mode (MDM) consist of switching alternatively between Reversed and Normal Phase operation during the experiment (once for DM and several times for MDM). In this paper, MDM has been applied to the chiral CCC separations of two racemic mixtures, (+/-)-N-(3,4-cis-3-decyl-1,2,3,4-tetrahydrophenanthren-4-yl)-3,5-dinitrobenzamide and N-(3,5-dinitrobenzoyl)-(+/-)-leucine, using (S)-naproxen N,N-diethylamide as chiral selector (CS). Although the behaviour of the two analytes differed, improved resolution factors were successfully obtained. Results are rationalized on the basis of the distinct partition behaviour of the CS/enantiomer complexes in the biphasic system. (C) 2009 Elsevier B.V. All rights reserved.
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