Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 34, Pages 6196-6203Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2009.06.081
Keywords
Molecular imprinting; Solid-phase extraction; Melamine; Feed; Milk
Funding
- National Science and Technology Project [2006BAD31 1306]
- South China Agricultural University
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A water compatible molecularly imprinted polymer (MIP) using cyromazine as a mimic template, methacrylic acid as the functional polymer and ethylene glycol dimethacrylate as the cross-linker was synthesized and used to extract melamine from feed and milk samples via a molecularly imprinted solid-phase extraction (MISPE) protocol. Optimum retention of melamine on the MISPE cartridge was achieved using methanol, and the interferences in the samples were effectively washed out. The binding capacity of the polymer toward melamine was found to be about 500 mu g of melamine/g of polymer. The recoveries of 2 mu g and 20 mu g melamine standard spiked into water extract of blank feeds and milk samples were between 83.4% and 103%. with relative standard deviation <5.6%.The cyromazine-MIP demonstrated high cross-reactivity for melamine and low affinity to cyanuric acid. The ionic bond interaction was regarded as the main factors that dominated the retention of the melamine on the MISPE cartridge. (c) 2009 Elsevier B.V. All rights reserved
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