Application of response surface method for optimization of dispersive liquid-liquid microextraction of water-soluble components of Rosa damascena Mill. essential oil

Dispersive liquid-liquid microextraction (DLLME) Coupled with gas chromatography-mass spectrometry (GC-MS) was applied for the determination of Rose water constituents. The effective parameters such as volume of extraction and disperser solvents, temperature, and salt effect were inspected by a full factorial design to identify important parameters and their interactions. It showed that salt addition had no effect on the efficiency. Next. a central composite design was applied to obtain optimum point of the important parameters. The optimal condition was obtained as 37.0 mu L for extractor. 0.42 mL for disperser and temperature for 48 degrees C. The main components that were extracted at the optimum point were benzeneethanol (24.87%), geraniol (23.07%), beta-citronellol (22.38%), nerol (8.48%), eugenol (5.98%) and linalool (5.62%). (C) 2008 Elsevier B.V. All rights reserved.