Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 26, Pages 5061-5068Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.04.077
Keywords
Beer; Carbonyl compounds; Derivatisation; In-solution; On-fibre; Solid-phase microextraction; Gas chromatography; Mass spectrometry
Funding
- Institute for the promotion of Innovation by Science and Technology in Flanders (IWT Flanders)
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Headspace solid-phase microextraction (SPME) followed by gas chromatography and mass spectrometry was applied for quantification of 41 chemically diverse carbonyl compounds in beer. Therefore, in-solution derivatisation with o-(2,3,4,5.6-pentafluorobenzyl)hydroxylamine (PFBHA) combined with SPME was optimised for fibre selection, PFBHA concentration, extraction temperature and time and ionic strength. Afterwards, the method was calibrated and validated successfully and extraction efficiency was compared to sampling with on-fibre derivatisation. In-solution derivatisation enabled the detection of several compounds that were poorly extracted with on-fibre derivatisation such as 5-hydroxymethylfurfural, acrolein, hydroxyacetone, acetoin, glyoxal and methylglyoxal. Others, especially (E)-2-nonenal, were extracted better with on-fibre derivatisation. (C) 2009 Elsevier B.V. All rights reserved.
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