Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 16, Pages 3443-3457Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.08.111
Keywords
Flavones; Phenolic compounds; Comprehensive two-dimensional liquid chromatography; Gradient elution; Porous-shell columns
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An almost orthogonal comprehensive two-dimensional liquid chromatography was developed for the separation of phenolic and flavone natural antioxidants by using combinations of a polyethylene glycol silica micro-column in the first dimension and a porous-shell fused-core C18 column in the second dimension. both in the reversed-phase mode. System orthogonality was improved using parallel gradients of acetonitrile in buffered mobile phase. A new approach was proposed to optimize matching segmented gradient profiles in the two dimensions. An algorithm was developed for automatic corrections of the shifts in retention in the second dimension induced by the parallel two-dimensional gradient operation technique. Using the porous-shell C18 column in the second dimension at elevated temperature (60 degrees C) and high pressure (480 bar)with optimized segmented profiles of the parallel gradients in the two dimension:, the overall separation time for comprehensive LC x LC was reduced to 30min. (C) 2008 Elsevier B.V. All rights reserved.
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