Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 47, Pages 8421-8425Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.10.014
Keywords
Boronate affinity; Capillary liquid chromatography; Capture; Glycoprotein; Monolithic column
Funding
- National Natural Science Foundation of China [20675038]
- 973 Program [2007CB914102]
- Ministry of Science and Technology of China [2008DFA01910]
- Natural Science Foundation of Jiangsu Province [KB2008258]
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Boronate affinity chromatography is an important tool for specific isolation of cis-diol-containing compounds such as glycoproteins, RNA and carbohydrates. Boronate functionalized monolithic capillaries have been recently developed for specific capture of cis-diol-containing small biomolecules, but the apparent hydrophobicity of the columns prevents them from specific capture of glycoproteins. In this paper, a hydrophilic boronate affinity monolithic capillary was prepared by in situ free radical polymerization, using 4-vinylphenylboronic acid (VPBA) and N, N'-methylenebisacrylamide (MBAA) as functional monomer and cross-linker, respectively. The prepared poly(VPBA-co-MBAA) monolithic capillary exhibited uniform open channel network and high density of accessible boronic acid. Due to the utilization of hydrophilic cross-linker, the prepared column was hydrophilic, allowing for specific capture of glycoproteins. (C) 2009 Elsevier B.V. All rights reserved.
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