Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 17, Pages 3646-3651Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.12.090
Keywords
Acidity constant; Capillary electrophoresis; pK(a); Internal standard; High-throughput method
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A new and fast method to determine acidity constants of monoprotic weak acids and bases by capillary zone electrophoresis based on the use of an internal standard (compound of similar nature and acidity constant as the analyte) has been developed. This method requires only two electrophoretic runs for the determination of an acidity constant: a first one at a pH where both analyte and internal standard are totally ionized, and a second one at another pH where both are partially ionized. Furthermore, the method is not pH dependent, so an accurate measure of the pH of the buffer solutions is not needed. The acidity constants of several phenols and amines have been measured using internal standards of known pK(a), obtaining a mean deviation of 0.05 pH units compared to the literature values. (c) 2009 Elsevier B.V. All rights reserved.
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