Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 27, Pages 5320-5326Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2009.05.012
Keywords
Enantiomer separation; Molecular imprinting; Monolithic column; Pressurized capillary electrochromatography; Recognition mechanism
Funding
- National High-Tech RD Program [2006AA09z161]
- National Key Technologies RD Program [2006BAK02A21]
- Science Foundation of Fujian Province [2007J0129]
- Key Science & Technology Project of Jujian Province [2007Y0062, 2008Y0301]
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Molecularly imprinted monolithic columns were prepared for chiral separation of tyrosine and its amino acid derivatives by in situ therm-initiated copolymerization of methacrylic acid, 4-vinylpyridine and ethylene glycol dimethacrylate. The enantiomers were rapidly separated on monolithic columns in less than 10 min by pressurized capillary electrochromatography (pCEC). The influences of several parameters such as the content of cross-linking monomer on the composition of the pre-polymerization mixture were systematically investigated. The influence of the pCEC conditions including the composition of the mobile phase was also optimized to obtain the good enantioseparation. It was found that in addition to molecularly imprinted recognition, chromatographic retention and electrophoretic migration play important roles in the retention and chiral recognition of molecularly imprinted polymer (MIP) columns. The cross-selectivity for similar amino acids and its derivatives were systematical investigated for understanding the recognition mechanism on the MIP monolithic columns. The results indicated that molecularly imprinted polymer recognizes the template molecule by its molecular shape defined binding cavity. (C) 2009 Elsevier B.V. All rights reserved.
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