Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 13, Pages 2671-2675Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.09.102
Keywords
Conjoint liquid chromatography (conjoint LC); IgG; Monolith; CIM disk; Protein G
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The aim of this study was to develop a chromatographic method, as a substitute for enzyme-linked immunosorbent assays, for the rapid and simultaneous detection of IgG, insulin, and transferrin present in a cell culture medium. Conjoint liquid chromatography (conjoint LC) using monolithic disks was applied for this purpose. An anion-exchange disk was combined with a Protein G affinity disk in a preparative HPLC system. IgG bound to the Protein G disk, whereas transferrin and insulin were captured on the quaternary ammonium (QA) disk. Using this method, it was possible to simultaneously determine the concentrations of IgG, transferrin, and insulin in the cell culture medium. Thus, conjoint LC could be used for the rapid and simultaneous detection of different proteins present in a cell culture medium. (C) 2008 Elsevier B.V. All rights reserved.
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