Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 49, Pages 8665-8670Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.10.027
Keywords
Transient isotachophoresis; Strong counter-electroosmotic flow; Open system; Oxidized and reduced glutathione; Tomato; Human serum
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Transient isotachophoresis (tITP) can improve the sensitivity of capillary electrophoresis (CE). In general, it was carried out under the condition of suppressed electroosmotic flow (EOF). However, some special conditions, such as extreme low pH background electrolyte and coating were needed to achieve the requirements of suppressed EOF. In this work, an approach of tITP under the strong counter-EOF in open system (counter-EOF-OTP) is presented for the rapid and sensitive preconcentrating the reduced glutathione (GSH) and the oxidized glutathione (GSSG) without modifying the capillary and the commercial CE instrument. The parameters of the experimental system, such as the concentration of leading electrolyte, the injected amount of terminating electrolyte and the injected pressure of sample were investigated in detail to understand the mechanism of counter-EOF-tITP. The sensitivity enhancement factors were of 320 for GSH and 280 for GSSG. In addition, the detection limit of 23.4 and 18.0 mu g L-1 for GSH and GSSG was achieved, respectively. The method's applicability was demonstrated by determining GSH and GSSG in tomato and human serum. (C) 2009 Elsevier B.V. All rights reserved.
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