Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 11, Pages 2185-2191Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.05.074
Keywords
Comprehensive two-dimensional chromatography; ILC column; Biological finger printing analysis; Longdan Xiegan Decoction; Traditional Chinese medicine
Funding
- CAS [KGCX2-SW-213-04]
- National Natural Science Foundation of China [30530840]
- National Basic Research Program [2006CB504704]
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A comprehensive two-dimensional HPLC system with an immobilized liposome chromatography (ILC) column in conjunction with all RP column (in tandem) was developed for the screening and analysis of the membrane-permeable Compounds in a traditional Chinese medicine prescription Longdan Xiegan Decoction (LXD). More than 50 components in LXD were resolved using the developed separation system. Eight flavonoids and two iridoids were identified interacting with the ILC column: a system that mimics biomembranes. The results show that the developed comprehensive two-dimensional chromatography system can be used for identifying membrane permeable natural products in complex matrixes such as extracts of traditional Chinese medicine prescriptions. (C) 2008 Published by Elsevier B.V.
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