Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 15, Pages 3398-3401Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.01.106
Keywords
Liquid chromatography; Fluorescence detection; Solid-phase extraction; Mixed bed; Biogenic amines; Wine samples
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Biogenic amines in wine usually are analyzed by high-performance liquid chromatography after direct derivatization. A method of isolation based on solid-phase extraction (SPE) with mixed-mode resins(Oasis MCX, reverse-phase and ion exchange) was developed. The different stages of the isolation process (loading, elution and washing) were optimized to obtain a simple procedure that yields a clean chromatogram. The relative standard deviation (%RSD) of the retention times and relative areas was less than 0.3% and 6%, respectively. Limits of quantification were lower than 0.16 mg L-1 for all the amines and the linear range of concentration was 0.16-8 mg L-1 for putrescine, cadaverine and tyramine, and up to 10 mg L-1 for histamine. (C) 2009 Elsevier B.V. All rights reserved.
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