Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1206, Issue 1, Pages 45-51Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.05.077
Keywords
Dielectrophoresis; Proteins; Microfluidics; Electrokinetics; Electrophoresis; Electroosmosis
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The present study demonstrates the manipulation of protein particles employing insulator-based dielectrophoresis (iDEP) and direct current (d.c.) electric fields. Fluorescently labeled bovine serum albumin (BSA) protein particles were concentrated inside a microchannel that contained an array of glass cylindrical insulating structures. d.c. electric fields were applied and the dielectrophoretic response of the particles was observed as a function of the suspending medium conductivity (25, 50 and 100 mu S/cm) and pH (8 and 9). It was shown that the magnitude of the applied electric field (700-1600V/cm) and suspending medium properties have a strong effect on the dielectrophoretic response of the protein particles. The results presented here are the first report on protein manipulation employing d.c.-iDEP. (C) 2008 Published by Elsevier B.V.
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