Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1177, Issue 2, Pages 272-281Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2007.07.083
Keywords
hydrophobic interaction chromatography; hydrophobic charge induction chromatography; mAb purification; resin pore size; salt and pH effects; capture and polish processes
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Recent efforts to improve hydrophobic interaction chromatography (HIC) for use in monoclonal antibody (mAb) purification have focused on two approaches: optimization of resin pore size to facilitate mAb mass transport, and use of novel hydrophobic charge induction (HCIC) mixed mode ligands that allow capture of mAbs under low salt conditions. We evaluated standard HIC and new generation HIC and HIC-related chromatography resins for mAb purification process efficiency and product quality both as isolated chromatography steps and in purification process trains. We find that HIC resins with optimized pore size have significantly improved binding capacity which can increase HIC purification unit operation efficiency. The HCIC Mercapto-Ethyl-Pyridine (MEP) resin, which shows a different salt impact trend and impurity resolution pattern from standard HIC resin, can not only capture mAb from crude CHO fermentation supernatant but also substantially enhance mAb purification process flow efficiency when serving as a polishing role. (C) 2007 Elsevier B.V. All rights reserved.
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