Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1189, Issue 1-2, Pages 398-405Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.10.080
Keywords
polyphenols; flavan-3-ol; proanthocyanidins; barley; malt; HPLC-ESI-MS
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The pattern of the monomeric and oligomeric flavan-3-ols for 10 barley varieties and the corresponding malts were identified and quantified using high-performance liquid chromatography-ultraviolet detection-electrospray ion trap mass spectrometry. The Folin-Ciocalteau and the vanillin spectrophotometric assays were used for the assessment of the total polyphenol and total flavan-3-ol content, respectively, and the antioxidant activity was determined as the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the ferricyanide reducing power. Catechin and prodelphinidin B3 were respectively the major monomeric and dimeric flavan-3-ols. Moreover, prodelphinidin B3 was shown to be the main contributor for the radical scavenging activity both for barley and malt. (c) 2007 Elsevier B.V. All rights reserved.
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