Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1191, Issue 1-2, Pages 2-20Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.10.079
Keywords
column selectivity; peak tailing; orthogonal separation; column stability; hydrophobic-subtraction model
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A total of 371 reversed-phase columns have now been characterized in terms of selectivity, based on five solute-column interactions (the hydrophobic-subtraction model). The present study illustrates the use of these data for interpreting peak-tailing and column stability. New insights are also provided concerning column selectivity as a function of ligand and silica type, and the selection of columns for orthogonal separations is re-examined. Some suggestions for the quality control of reversed-phase columns during manufacture are offered. (c) 2007 Elsevier B.V. All rights reserved.
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