Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1207, Issue 1-2, Pages 175-180Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.08.062
Keywords
Poly(dimethylsiloxane); Microchip; Micellar electrokinetic chromatography; Protein; Functionalized; Ionic liquid
Funding
- National Natural Science Foundation of China [20575063, 2006AA020701, 2007CB714500]
- Chinese Academy of Sciences [KJCX2.YW.HO11]
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Herein, one water-soluble functionalized ionic liquid (IL), 1-butyl-3-methylimidazolium dodecanesulfonate (BAS), was designed, investigated and successfully applied to microchip micellar electrokinetic chromatography (MEKC) construction. It possessed the properties of both IL and surfactant. A fairly stable pH value similar to 7.4, which was fit to pH values of general biological buffers, was nicely placed at the optimum concentration of 20 mM BAS solution. While applying BAS solution as running buffer in poly(dimethylsiloxane) (PDMS) microfluidic systems, significantly enhanced electroosmotic flow (8-fold) and resolutions between analytes were obtained than that using other supporting electrolytes or surfactants. Pure BAS solution could conveniently complete the task of MEKC establishment: not only the ionic strength of the running buffer was guaranteed, but also the analytes adsorption to PDMS surface was conquered, as was demonstrated in the sensitive determination of fluorescent dyes and well-separated protein mixtures. (C) 2008 Elsevier B.V. All rights reserved.
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