Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1201, Issue 1, Pages 43-47Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.06.002
Keywords
anthocyanin; flavonol; flavonoid; HPLC; mass spectrometry
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A limitation of large-scale viticultural trials is the time and cost of comprehensive compositional analysis of the fruit by high-performance liquid chromatography (HPLC). In addition, separate methods have generally been required to identify and quantify different classes of metabolites. To address these shortcomings a reversed-phase HPLC method was developed to simultaneously separate the anthocyanins and flavonols present in grape skins. The method employs a methanol and water gradient acidified with 10% formic acid with a run-time of 48 min including re-equilibration. Identity of anthocyamins and flavonols in Shiraz (Vitis vinifera L.) skin was confirmed by mass spectral analysis. (c) 2008 Elsevier B.V. All rights reserved.
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