Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1198, Issue -, Pages 59-66Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.05.059
Keywords
molecularly imprinted solid-phase microextraction; molecularly imprinted polymer-sensor; ascorbic acid; differential pulse; cathodic stripping voltammetry; blood serum
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Clinical manifestations owing to ascorbic acid deficiency demand an easy-to-use, rapid, robust and inexpensive technique, which can measure serum ascorbic acid at ultratrace level to attend the problem of hypovitaminosis C and acute cases of scurvy. In the present work, a novel molecularly imprinted polymer (MIP)-coated solid-phase microextraction (SPME) fiber that could be coupled to a complementary MIPsensor was prepared with ascorbic acid as a template molecule. The characteristics and applications of this fiber were investigated. The same MIP receptor for both SPME and the corresponding sensor was able to enhance the preconcentration of analyte substantially so as to attain the stringent level of sensitivity in highly diluted aqueous, blood serum and pharmaceutical samples. The extraction yield of ascorbic acid (AA) with the MIP-coated fiber was found to be quantitative (detection limit 0.0396 ng mL(-1), RSD = 2.3%. S/N = 3) in aqueous samples without any problem of non-specific false positive results and cross-reactivity. (c) 2008 Elsevier B.V. All rights reserved.
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