Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1184, Issue 1-2, Pages 107-130Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.11.113
Keywords
peak capacity; resolution; gradient chromatography; peak compression; peak overlap; peptides; Oligonucleotides; ion-exchange chromatography; size-exclusion chromatography
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The currently existing knowledge about peak capacity in unidimensional separations is reviewed. The majority of the paper is dedicated to reversed-phase gradient chromatography, covering specific techniques as well as the subject of peak compression. Other sections deal with peak capacity in isocratic chromatography, size-exclusion chromatography and ion-exchange chromatography. An important topic is the limitation of the separation power and the meaning of the concept of peak capacity for real applications. (c) 2007 Elsevier B.V. All rights reserved.
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