Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1209, Issue 1-2, Pages 212-219Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.08.114
Keywords
Fatty acid methyl esters; Gas-liquid chromatography; Forage plants
Funding
- Faculdade de Ciencias
- da Universidade de Lisboa, Portugal [SFRH/BD/37793/2007]
- Fundacao para Ciencia e a Tecnologia (FCT) [POCI/CVr/61202/2004]
- Ministerio da Uncia, Tecnologia e Ensino Superior, Portugal
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Direct transesterification (DT) and solvent extraction with acid or basic derivatization procedure for fatty acid (FA) analysis in herbage were compared. The highest total FA, alpha-linolenic and linoleic acid contents were obtained with DT. However, DT also produced the highest amount of interfering compounds, identified as phytadienes and sugar derivative products, which may co-elute with FA. An additional step based on solid-phase extraction to produce clean samples was proposed. This procedure is simple and gives good recoveries for the FA fortified samples. Additionally, structural characterization of 16:1 trans-3 was conducted by covalent adduct chemical ionization tandem mass spectrometry. (C) 2008 Elsevier B.V. All rights reserved.
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