Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1209, Issue 1-2, Pages 253-259Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.08.087
Keywords
CSEI-sweeping-MEKC; Selective serotonin reuptake inhibitor; On-line preconcentration; Antidepressant drug; MEKC
Funding
- National Science Council, Taiwan [NSC 95-2113-M-009-033-MY3]
Ask authors/readers for more resources
We have employed a rapid and highly efficient on-line preconcentration method, cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography (CSEI-sweeping-MEKC), for the analysis of selective serotonin reuptake inhibitors (SSRIs) of antidepressant drugs. We monitored the effects of several of the CSEI-sweeping-MEKC parameters - including the pH, the concentrations of high-conductivity buffer (HCB), sodium dodecyl sulfate (SDS). and organic modifier, the injection length of the HCB, and the injection time of the sample - to optimize the separation process. The optimal background electrolyte was 50 mM citric acid/disodium hydrogenphosphate buffer (pH 2.2) containing 100 mM SDS and 22% isopropyl alcohol. The sensitivity enhancements of the SSRIs sertraline, fluoxetine, paroxetine, fluvoxamine, and citalopram ranged from 5.7 x 10(4) to 1.2 x 10(5); the coefficients of determination exceeded 0.9938 and the relative standard deviations of the peak heights were less than 3.2%; the detection limits ranged from 0.056 to 0.22 ng/mL We employed the optimal conditions to analyze these five SSRIs in a plasma sample prepared using solid-phase extraction (SPE) to minimize the influence of the matrix. Although the limits of detection of the SSRIs in human plasma were higher than those in pure water, this present technique is more sensitive than other, more-conventional methods. The recovery of the SPE extraction efficiency was satisfactory (up to 89%). Our findings suggest that, under the optimal conditions, the CSEI-sweeping-MEKC method can be used successfully to determine these five SSRIs in human plasma. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available