Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1193, Issue 1-2, Pages 92-96Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.04.002
Keywords
A-type and B-type trichothecenes; deepoxydeoxynivalenol; digestates of biogas production; gas chromatography-mass spectrometry; high-performance liquid chromatography; post-column derivatisation; sample preparation
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A sensitive and selective method for the simultaneous determination of a spectrum of trichothecenes in residuals of biogas production has been developed. It comprises sample clean-up by liquid/liquid partition for digested manure and solid phase extraction for digested solid phase. Quantification of A- and B-type trichothecenes as their trifluoroacetyl derivatives is performed by gas chromatography mass spectrometry (GC/MS), that of B-type trichothecenes alternatively by high-performance liquid chromatography (HPLC). Fluorescence detection (FLD) after post-column derivatisation using methylacetoacetate and ammonium acetate after alkaline decomposition of toxins was applied. Detection limits in digestates were between 1 and 30 mu g/l and 20 and 50 mu g/l for GC/MS and HPLC/FLD, respectively. Recovery rates were between 52 and 129% for GC/MS detection with the exception of T-2 tetraol with 22%, and between 56 and 123% for HPLC/FLD. (c) 2008 Elsevier B.V. All rights reserved.
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