Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1190, Issue 1-2, Pages 182-190Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.02.114
Keywords
alprazolam; impurity profile; orthogonality; two-dimensional liquid chromatography; ultra performance liquid chromatography
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Separation orthogonality has been explored with respect to comprehensive two-dimensional liquid chromatography (2D-LC) for different reversed-phase stationary phases. The outcome of this study points out an SB-CN x BEH-C18 combination, used in the first and the second dimension, respectively, as the most orthogonal chromatographic system for the samples assayed. The present investigation reports the employment of an ultra high pressure liquid chromatography system (UPLC) as the second chromatographic dimension, increasing the sensitivity and the speed, completing the whole chromatographic separation in a reasonable time frame. Finally, an off-line 2D-LC method with diode array detection based on the UPLC has been optimized, allowing the separation and minimizing the run time. SB-CN and BEH-C18 were employed as first and second dimension, respectively, with gradient elution applied in each dimension. Alprazolam degraded tablets were studied as a proof of concept of the utility of this type of setups for impurity profiling of complex samples. (C) 2008 Elsevier BY. All rights reserved.
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