Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1187, Issue 1-2, Pages 111-118Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.02.010
Keywords
carbohydrate; water deficit; early stress; severe stress; porous graphitic carbon; mass spectrometry; Lupinus albus; stem
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This work reports the development and application of a negative ion mode online LC-ESI-MS method for studying the effect of water deficit on the carbohydrate content of Lupinus albus stems, using a porous graphitic carbon (PGC) stationary phase and an ion trap mass spectrometer. Using this method, separation and detection of several water soluble carbohydrates, ranging from mono-, di-, and oligosaccharides (raffinose, stachyose, and verbascose) to sugar alcohols was achieved in approximately 10 min. This on-line PGC-LC-ESI-MS method shows good linearity with correlation coefficients R-2 > 0.99, selectivity, short analysis time, and limits of detection (LOD) ranging from 0.4 to 9 pmol for sugars and 4-20 pmol for sugar alcohols. This PGC-LC-ESI-MS method is sensitive and allowed us to detect even small alterations in carbohydrate levels in L. albus stems that resulted from a mild/early water deficit (nmol g(-1) DW). This paper describes details of our method and its application to the quantitative analysis of water soluble underivatised carbohydrates extracted from L albus stem tissues that have been subjected to early and severe water deficit conditions, followed by a rewatering period. (c) 2008 Elsevier B.V. All rights reserved.
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