Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1208, Issue 1-2, Pages 133-140Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.08.079
Keywords
Two-dimensional liquid chromatography; Cyclodextrin; 2D-RPLC/HILIC; Orthogonality evaluation; Low-abundant component; Traditional Chinese medicine
Funding
- Knowledge Innovating Project [KGCX2-SW-213]
- Science and Technology Plan of Liaoning Province [2006226002]
- Knowledge Innovation Program of DICP
- CAS [K2006A4, K2006A3]
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A novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography (2D-RPLC/HILIC) system is developed with the introduction of the click beta-cyclodextrin (beta-CD) stationary phase. The offline system shows excellent abilities for the separation of polar and medium-polarity components in traditional Chinese medicine (TCM). It facilitates us not only to separate components that cannot be resolved by uni-dimensional chromatography, but also to achieve much more efficient detection of components with low abundance. The orthogonality of this new system is excellent. A simple geometric approach is developed to characterize the practical orthogonality of 2D-LC in the analysis of complex unknown samples. The mathematical characterization results are in good accordance with the experimental findings, and both demonstrate that the proposed 2D-RPLC/HILIC system is a powerful tool for the separation of polar and medium-polarity complex samples. This system may have a great potential for the separation of peptides and metabolomic compounds with similar polarity. (C) 2008 Elsevier B.V. All rights reserved.
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