Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1214, Issue 1-2, Pages 151-156Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.10.068
Keywords
Enantiomeric separation; Amino acids; Diastereomeric; N-ethoxycarbonyl/(S)-1-phenylethylamides; Gas chromatography-mass spectrometry in selected ion monitoring mode; Beans; D-Pipecolic acid
Funding
- Basic Research Program of the Korea Science and Engineering Foundation, South Korea [2006-04027]
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N-Ethoxycarbonylation was combined with (S)-1-phenylethylamidation for enantioseparation of amino acids by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) on achiral capillary columns. The method provided complete enantioseparations of 12 amino acids as diastereomeric N-ethoxycarbonyl/(S)-1-phenylethylamides with exceptional resolutions for proline (R-s >= 9.9) and pipecolic acid (R-s >= 10.2). GC-MS analysis in selected ion monitoring mode employing standard addition method, facilitated quantitation of D-pipecolic acid in kidney bean (0.95 mu g/10 mg) and adzuki bean (0.14 mu g/10 mg). The peak area ratios indicated that they had the identical chiral composition at 2.5% for D-pipecolic acid and 97.5% for L-pipecolic acid. 2008 Elsevier B.V. All rights reserved.
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