Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1202, Issue 2, Pages 111-117Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.06.021
Keywords
deoxynivalenol; mycotoxin; immuno-ultrafiltration; sample clean-up
Funding
- Austrian Science Foundation [L98-N19]
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This paper describes the development of a highly selective analytical method for the determination of deoxynivalenol (DON) in maize. The developed method is based on immuno-ultrafiltration (IUF) and is the first application of IUF as a clean-up strategy in food analysis. Quantification of DON was carried out by high-performance liquid chromatography with ultraviolet detection. In contrast to immunoaffinity chromatography, in IUF the antibodies are not bound to a solid support material but used in free form, thus making it possible to avoid the critical immobilisation step. Sample clean-up by IUF proved to be as selective as clean-up using commercially available immunoaffinity columns. The limit of detection (S/N = 3)of the analytical method was found to be 74 ng DON/g maize. Repeated analysis of a certified maize reference material on four different days resulted in a mean recovery of 93% with a standard deviation of 10%. (C) 2008 Elsevier B.V. All rights reserved.
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