Journal
JOURNAL OF CHROMATOGRAPHIC SCIENCE
Volume 53, Issue 5, Pages 725-729Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/chromsci/bmu114
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Funding
- National Natural Science Foundation of China [21105090]
- Department of Education of Zhejiang Province of China [pd2013031]
- Innovative Program for the Undergraduates of Zhejiang University of Technology [2013059]
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The bioactive iridoid component catalpol was successfully separated by high-speed countercurrent chromatography with high purity from the partially purified crude extract of Rehmannia glutinosa. A polar two-phase solvent system composed of ethyl acetate-n-butanolwater (2: 1: 3, v/v/v) was selected by thin-layer chromatography and run on a preparative scale where the lower aqueous phase was used as the mobile phase with a head-to-tail elution mode. A 105 mg quantity of the partially purified sample containing 39.2% catalpol was loaded on a 270-mL capacity high-speed countercurrent separation column, yielding 35 mg of catalpol at 95.6% purity. The chemical structure of catalpol was determined by comparison with the high-performance liquid chromatography retention time of standard substance as well as the H-1 NMR spectrum.
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