Journal
JOURNAL OF CHROMATOGRAPHIC SCIENCE
Volume 53, Issue 5, Pages 836-840Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/chromsci/bmu108
Keywords
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Funding
- National Research Foundation (NRF) of Korea - Ministry of Education, Science and Technology [2014-002046]
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Three flavones (quercetin, myricetin and amentoflavone) were extracted from Chamaecyparis obtusa leaves using deep eutectic solvents (DESs) as additives to conventional extractions solvents. Sixteen DESs were synthesized from different salts and hydrogen bond donors. In addition, C. obtusa was extracted under optimal conditions of methanol as the solvent in the heating process (60 degrees C) for 120 min at a solid/liquid ratio of 80%. Under these optimal conditions, a good linear relationship was observed at analyte concentrations ranging from 5.0 to 200.0 mu g/mL (R-2 > 0.999). The extraction recovery ranged from 96.7 to 103.3% with the inter-and intraday relative standard deviations of <4.97%. Under the optimal conditions, from C. obtusa, the quantities of quercetin, myricetin and amentoflavone extracted were 325.90, 8.66 and 50.34 mu g/mL, respectively. Overall, DESs are expected to have a wide range of applications.
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