Journal
JOURNAL OF CHROMATOGRAPHIC SCIENCE
Volume 52, Issue 9, Pages 1043-1050Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/chromsci/bmt157
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Andrographis paniculata (Burm. f.) wall. ex Nees (Acanthaceae) or Kalmegh is an important medicinal plant finding uses in many Ayurvedic formulations. Diterpenoid compounds andrographolides (APs) are the main bioactive phytochemicals present in leaves and herbage of A. paniculata. The efficiency of supercritical fluid extraction (SFE) using carbon dioxide was compared with the solid-liquid extraction techniques such as solvent extraction, ultrasoundassisted solvent extraction and microwave-assisted solvent extraction with methanol, water and methanol-water as solvents. Also a rapid and validated reverse-phase high-performance liquid chromatography-diode array detection method was developed for the simultaneous determination of the three biologically active compounds, AP, neoandrographolide and andrograpanin, in the extracts of A. paniculata. Under the best SFE conditions tested for diterpenoids, which involved extraction at 60 degrees C and 100 bar, the extractive efficiencies were 132 and 22 mu g/g for AP and neoandrographolide, respectively. The modifier percentage significantly affected the extraction efficiency.
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