Journal
NEURON
Volume 88, Issue 2, Pages 277-288Publisher
CELL PRESS
DOI: 10.1016/j.neuron.2015.09.043
Keywords
-
Categories
Funding
- Wellcome Trust Principal
- Medical Research Council (UK)
- Biology and Biotechnology Research Council (UK)
- European Research Council Advanced Grant
- FP7 ITN EXTRABRAIN grant
- Russian Science Foundation [15-14-30000]
- EMBO Fellowship
- BBSRC [BB/J001473/1] Funding Source: UKRI
- MRC [G0600368] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J001473/1] Funding Source: researchfish
- Medical Research Council [G0600368] Funding Source: researchfish
Ask authors/readers for more resources
Maintaining low intracellular calcium is essential to the functioning of brain cells, yet the phenomenology and mechanisms involved remain an enigma. We have advanced a two-photon excitation time-resolved imaging technique, which exploits high sensitivity of the OGB-1 fluorescence lifetime to nanomolar Ca2+ concentration ([Ca2+]) and enables a high data acquisition rate in situ. The [Ca2+] readout is not affected by dye concentration, light scattering, photobleaching, micro-viscosity, temperature, or the main known concomitants of cellular activity. In quiescent tissue, standard whole-cell configuration has little effect on resting [Ca2+] inside neuronal dendrites or inside astroglia dye-filled via gap junctions. Mapping basal [Ca2+] in neurons and astrocytes with submicron resolution unveils heterogeneous concentration landscapes that depend on age and preceding activity. The rich information content represented by such landscapes in acute slices and in vivo promises to unveil the hitherto unexplored, potentially fundamental aspects of brain cell physiology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available