Journal
JOURNAL OF CHEMICAL EDUCATION
Volume 91, Issue 6, Pages 902-907Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ed400728a
Keywords
Upper-Division Undergraduate; Analytical Chemistry; Bioanalytical Chemistry; Laboratory Instruction; Hands-On Learning/Manipulatives; Separation Science; Electrophoresis; Fluorescence Spectroscopy; Microscale Lab; Nucleic Acids/DNA/RNA
Funding
- Natural Sciences and Engineering Research Council of Canada
- Ontario Ministry of Training, Colleges and Universities
- Department of Chemical and Physical Sciences of the University of Toronto Mississauga
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An advanced analytical chemistry undergraduate laboratory module on microfluidics that spans 4 weeks (4 h per week) is presented. The laboratory module focuses on comprehensive experiential learning of microfluidic device fabrication and the core characteristics of microfluidic devices as they pertain to fluid flow and the manipulation of samples. Experiments include the cleanroom-free fabrication of glass-polydimethylsiloxane (PDMS) microfluidic devices by use of thermoplastic molds and soft lithography, determination of the zeta potential at the microchannel walls, investigations of electroosmotic flow, determination of Pedet numbers, and diffusion coefficients based on diffusion-limited reagent mixing. The module concludes with the development of a microchannel electrophoresis lab-on-a-chip device with in-line fluorimetric detection for the separation of a DNA ladder. This laboratory module has been offered for two consecutive years and has received favorable student feedback.
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