Journal
JOURNAL OF CHEMICAL EDUCATION
Volume 90, Issue 9, Pages 1255-1256Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ed300390j
Keywords
Upper-Division Undergraduate; Biochemistry; Laboratory Instruction; Hands-On Learning/Manipulatives; Electrophoresis; Quantitative Analysis
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A variety of laboratory experiments have been published in this Journal describing the use of polyacrylamide gel electrophoresis to investigate different properties of proteins, including determination of the subunit composition of individual proteins and molar mass. The experiment described extends the capabilities of this experimental technique and provides a mechanism to determine the concentration of a single protein from a heterogeneous mixture.
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