Journal
JOURNAL OF CHEMICAL ECOLOGY
Volume 34, Issue 11, Pages 1422-1429Publisher
SPRINGER
DOI: 10.1007/s10886-008-9555-7
Keywords
Janthinobacterium lividum; Plethodon cinereus; Batrachochytrium dendrobatidis; Beneficial bacteria; Violacein; Indole-3-carboxaldehyde
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Funding
- Research Corporation Cottrell College Science Award (KPCM)
- Thomas F. Jeffress and Kate Miller Jeffress Memorial Trust (KPCM)
- National Science Foundation Research in Undergraduate Institutions (RNII) [0640373]
- James Madison University
- Mass Spectrometry Facility at Harvard University (Cambridge, MA, USA)
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Disease has spurred declines in global amphibian populations. In particular, the fungal pathogen Batrachochytrium dendrobatidis has decimated amphibian diversity in some areas unaffected by habitat loss. However, there is little evidence to explain how some amphibian species persist despite infection or even clear the pathogen beyond detection. One hypothesis is that certain bacterial symbionts on the skin of amphibians inhibit the growth of the pathogen. An antifungal strain of Janthinobacterium lividum, isolated from the skin of the red-backed salamander Plethodon cinereus, produces antifungal metabolites at concentrations lethal to B. dendrobatidis. Antifungal metabolites were identified by using reversed phase high performance liquid chromatography, high resolution mass spectrometry, nuclear magnetic resonance, and UV-Vis spectroscopy and tested for efficacy of inhibiting the pathogen. Two metabolites, indole-3-carboxaldehyde and violacein, inhibited the pathogen's growth at relatively low concentrations (68.9 and 1.82 mu M, respectively). Analysis of fresh salamander skin confirmed the presence of J. lividum and its metabolites on the skin of host salamanders in concentrations high enough to hinder or kill the pathogen (51 and 207 mu M, respectively). These results support the hypothesis that cutaneous, mutualistic bacteria play a role in amphibian resistance to fungal disease. Exploitation of this biological process may provide long-term resistance to B. dendrobatidis for vulnerable amphibians and serve as a model for managing future emerging diseases in wildlife populations.
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