Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 35, Issue 1, Pages 121-130Publisher
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2014.185
Keywords
cell death; excitotoxicity; hyperpolarization; ischemic tolerance; Na+ channels; neurotrophins
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Funding
- American Heart Association [12POST12090006, 13POST16720001]
- National Institutes of Health [NS45676, NS054147, NS34773]
- Miami Evelyn F. McKnight Brain Institute
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R55NS034773, R01NS054147, R29NS034773, R01NS045676, R01NS034773] Funding Source: NIH RePORTER
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Ischemic preconditioning (IPC) via protein kinase C epsilon (PKC epsilon) activation induces neuroprotection against lethal ischemia. Brain-derived neurotrophic factor (BDNF) is a pro-survival signaling molecule that modulates synaptic plasticity and neurogenesis. Interestingly, BDNF mRNA expression increases after IPC. In this study, we investigated whether IPC or pharmacological preconditioning (PKC epsilon activation) promoted BDNF-induced neuroprotection, if neuroprotection by IPC or PKC epsilon activation altered neuronal excitability, and whether these changes were BDNF-mediated. We used both in vitro (hippocampal organotypic cultures and cortical neuronal-glial cocultures) and in vivo (acute hippocampal slices 48 hours after preconditioning) models of IPC or PKC epsilon activation. BDNF protein expression increased 24 to 48 hours after preconditioning, where inhibition of the BDNF Trk receptors abolished neuroprotection against oxygen and glucose deprivation (OGD) in vitro. In addition, there was a significant decrease in neuronal firing frequency and increase in threshold potential 48 hours after preconditioning in vivo, where this threshold modulation was dependent on BDNF activation of Trk receptors in excitatory cortical neurons. In addition, 48 hours after PKC epsilon activation in vivo, the onset of anoxic depolarization during OGD was significantly delayed in hippocampal slices. Overall, these results suggest that after IPC or PKC epsilon activation, there are BDNF-dependent electrophysiologic modifications that lead to neuroprotection.
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