Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 32, Issue 6, Pages 938-951Publisher
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2012.42
Keywords
cerebral blood volume; forepaw electrical stimulation; hemodynamic response; hemoglobin oxygen saturation; total hemoglobin concentration; transcranial photoacoustic microscopy
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Funding
- National Science Council, ROC [97-2221-E-007-084-MY3, 96-2220-E-009-029, 97-2220-E-009-029, 99-2221-E-009-154, NSC 99-2911-I-009-101, 100-2911-I-009-101, 100-2321-B-009-001]
- National Tsing Hua University [98N2531E1]
- Ministry of Education, Taiwan
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Optical imaging of changes in total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel functional photoacoustic microscopy technique to probe the responses of single cortical vessels to left forepaw electrical stimulation in mice with intact skulls. Functional changes in HbT, CBV, and SO2 in the superior sagittal sinus and different-sized arterioles from the anterior cerebral artery system were bilaterally imaged with unambiguous 36x65-mu m(2) spatial resolution. In addition, an early decrease of SO2 in single blood vessels during activation (i.e., 'the initial dip') was observed. Our results indicate that the initial dip occurred specifically in small arterioles of activated regions but not in large veins. This technique complements other existing imaging approaches for the investigation of the hemodynamic responses in single cerebral blood vessels. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 938-951; doi:10.1038/jcbfm.2012.42; published online 4 April 2012
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