Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 32, Issue 12, Pages 2108-2113Publisher
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2012.136
Keywords
acetate; dynamic nuclear polarization; hyperpolarization; real-time metabolism; TCA cycle; 2-oxoglutarate
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Funding
- Swiss National Science Foundation [200020_124901, PP00P2_133562, 31003A_131087]
- National Competence Center in Biomedical Imaging
- Centre d'Imagerie BioMedicale of the UNIL
- UNIGE
- HUG
- CHUV
- EPFL
- Leenards Foundation
- Jeantet Foundation
- Swiss National Science Foundation (SNF) [200020_124901] Funding Source: Swiss National Science Foundation (SNF)
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The Krebs (or tricarboxylic acid (TCA)) cycle has a central role in the regulation of brain energy regulation and metabolism, yet brain TCA cycle intermediates have never been directly detected in vivo. This study reports the first direct in vivo observation of a TCA cycle intermediate in intact brain, namely, 2-oxoglutarate, a key biomolecule connecting metabolism to neuronal activity. Our observation reveals important information about in vivo biochemical processes hitherto considered undetectable. In particular, it provides direct evidence that transport across the inner mitochondria membrane is rate limiting in the brain. The hyperpolarized magnetic resonance protocol designed for this study opens the way to direct and real-time studies of TCA cycle kinetics. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 2108-2113; doi:10.1038/jcbfm.2012.136; published online 19 September 2012
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