Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 31, Issue 11, Pages 2152-2159Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/jcbfm.2011.112
Keywords
endogenous neuroprotection; lentiviral microRNA delivery; oxygen-glucose deprivation; SUMO conjugation
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Funding
- European Union [201024]
- European Union (European Stroke Network) [202213]
- German Research Foundation [TR43, DFG-EXC 257, KFO213 (HA5741)]
- VolkswagenStiftung
- Bundesministerium fur Bildung und Forschung (Center for Stroke Research Berlin)
- National Institutes of Health [HL095552]
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Small ubiquitin-like modifier (SUMO) 2/3 but not SUMO1 conjugation is activated after transient cerebral ischemia. To investigate its function, we blocked neuronal SUMO2/3 translation through lentiviral microRNA delivery in primary cortical neurons. Viability was unaffected by SUMO2/3 silencing unless neurons were stressed by transient oxygen-glucose deprivation (OGD). Both 15 and 45 minutes of OGD were tolerated by control microRNA-expressing neurons but damaged >60% of neurons expressing SUMO2/3 microRNA. Damaging OGD (75 minutes) increased neuronal loss to 54% (control microRNA) and to 99% (SUMO2/3 microRNA). This suggests that activation of SUMO2/3 conjugation is an endogenous neuroprotective stress response. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 2152-2159; doi:10.1038/jcbfm.2011.112; published online 24 August 2011
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