Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 29, Issue 7, Pages 1240-1250Publisher
SAGE PUBLICATIONS INC
DOI: 10.1038/jcbfm.2009.55
Keywords
chain migration; doublecortin (DCX); enhanced green fluorescent protein (eGFP); neuroblast; stroke; subventricular zone (SVZ)
Categories
Funding
- NHLBI NIH HHS [R01HL 64766, R01 HL064766-08, R01 HL064766] Funding Source: Medline
- NINDS NIH HHS [P01 NS042345, P50 NS023393, P01 NS42345, P50 NS23392, P01 NS042345-050003, P50 NS023393-19A10001] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL064766] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS023392, P50NS023393, P01NS042345] Funding Source: NIH RePORTER
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The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 +/- 1.04 mu m/h, which was significantly faster (P<0.01) than the speed of the cells in the SVZ (17.98 +/- 0.57 mu m/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes. Journal of Cerebral Blood Flow & Metabolism (2009) 29, 1240-1250; doi: 10.1038/jcbfm.2009.55; published online 13 May 2009
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