Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 29, Issue 11, Pages 1760-1763Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/jcbfm.2009.101
Keywords
blood-brain barrier; endothelium; pgp-1
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The ABC-transporter, p-glycoprotein-1 (pgp-1), is expressed on brain endothelium and is reported to be induced by several cytotoxic drugs, which are themselves substrates of pgp-1. Pgp-1 was increased on a human brain endothelial cell line (hCMEC/D3) after treatment with puromycin or verapamil. However, flow cytometry showed that the apparent upregulation caused by puromycin was not because of a global increase in expression levels, but selective cell death of a subpopulation of endothelium expressing the lowest levels of pgp-1. If a cytotoxic substrate of pgp-1 increases pgp-1 expression in vitro, it can easily be misinterpreted as a transcriptional activator of pgp-1. Journal of Cerebral Blood Flow & Metabolism (2009) 29, 1760-1763; doi:10.1038/jcbfm.2009.101; published online 29 July 2009
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