Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 29, Issue 1, Pages 108-118Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/jcbfm.2008.102
Keywords
brain glucose metabolism; glutamate-glutamine neurotransmitter cycling; magnetic resonance spectroscopy; (13)C isotopes
Categories
Funding
- NIH
- NIMH [NS37527, DK27121]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK027121] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [Z01MH002803] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS037527] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Over the last decade (13)C magnetic resonance spectroscopy ((13)C MRS) combined with the infusion of [1-(13)C] glucose has been used to measure the cerebral rate of the glutamate-glutamine cycle (V(cyc)). However, the effect of the astroglial label dilution pathways on the accuracy and precision of the (13)C MRS measurement of V(cyc) has not been evaluated or realized. In this report, we use the numerical Monte Carlo method to study the effect of astroglial dilution on the reliability of extracting Vcyc using the neuronal-astroglial two-compartment metabolic model and [1-(13)C] glucose infusion. The results show that omission of the astroglial dilution flux leads to a large loss in the sensitivity of the glutamine turnover curve to V(cyc). When the measured isotopic dilution of cerebral glutamine is accounted for in the analysis, the value of V(cyc) can be precisely and accurately determined.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available