4.5 Article

Localization of iron in rice grain using synchrotron X-ray fluorescence microscopy and high resolution secondary ion mass spectrometry

Journal

JOURNAL OF CEREAL SCIENCE
Volume 59, Issue 2, Pages 173-180

Publisher

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jcs.2013.12.006

Keywords

Biofortification; Phytate; IP6; Bioavailability

Funding

  1. Australian Research Council [LP0883746]
  2. HarvestPlus Challenge Program
  3. EPSRC Doctoral Training Account studentship
  4. Engineering and Physical Sciences Research Council [EP/I026584/1] Funding Source: researchfish
  5. EPSRC [EP/I026584/1] Funding Source: UKRI
  6. Australian Research Council [LP0883746] Funding Source: Australian Research Council

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Cereal crops accumulate low levels of iron (Fe) of which only a small fraction (5-10%) is bioavailable in human diets. Extensive co-localization of Fe in outer grain tissues with phytic acid, a strong chelator of metal ions, results in the formation of insoluble complexes that cannot be digested by humans. Here we describe the use of synchrotron X-ray fluorescence microscopy (XFM) and high resolution secondary ion mass spectrometry (NanoSIMS) to map the distribution of Fe, zinc (Zn), phosphorus (P) and other elements in the aleurone and subaleurone layers of mature grain from wild-type and an Fe-enriched line of rice (Olyza sativa L.). The results obtained from both XFM and NanoSIMS indicated that most Fe was co-localized with P (indicative of phytic acid) in the aleurone layer but that a small amount of Fe, often present as hotspots, extended further into the subaleurone and outer endosperm in a pattern that was not co-localized with P. We hypothesize that Fe in subaleurone and outer endosperm layers of rice grain could be bound to low molecular weight chelators such as nicotianamine and/or deoxymugineic acid. Crown Copyright (C) 2014 Published by Elsevier Ltd. All rights reserved.

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