Journal
JOURNAL OF CEREAL SCIENCE
Volume 56, Issue 3, Pages 615-622Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jcs.2012.08.016
Keywords
Proteome; Albumins; Globulins; 2D-DIGE; Gene expression
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Funding
- National Natural Science Foundation of China [30830072, 31101145]
- Chinese Ministry of Science and Technology [2009CB118303]
- National Key Project for Transgenic Crops of China [2011ZX08009-003-004, 2011ZX08002-004]
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Proteome Dynamics of albumins and globulins during grain development of elite Chinese wheat cultivar Xiaoyan 6 were studied. A total of 210 differentially expressed protein spots were recognized by two-dimensional differential gel electrophoresis (2D-DIGE) and 146 of these that represented 89 unique proteins were identified by MALDI-TOF mass spectrometry. Four protein expression patterns were observed across five grain developmental stages. Among the identified proteins, more than 80% were enzymes in eight functional categories, including carbohydrate metabolism (27%), protein metabolism (27%), stress/defense/detoxification (11%), cell metabolism (6%), transcription/translation (4%), nitrogen metabolism (4%), photosynthesis (4%), and signal transduction (1%). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 18 representative genes demonstrated that about two thirds of the genes displayed different expression profiles between the transcriptional and translational stages. (C) 2012 Elsevier Ltd. All rights reserved.
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