Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6

Proteome Dynamics of albumins and globulins during grain development of elite Chinese wheat cultivar Xiaoyan 6 were studied. A total of 210 differentially expressed protein spots were recognized by two-dimensional differential gel electrophoresis (2D-DIGE) and 146 of these that represented 89 unique proteins were identified by MALDI-TOF mass spectrometry. Four protein expression patterns were observed across five grain developmental stages. Among the identified proteins, more than 80% were enzymes in eight functional categories, including carbohydrate metabolism (27%), protein metabolism (27%), stress/defense/detoxification (11%), cell metabolism (6%), transcription/translation (4%), nitrogen metabolism (4%), photosynthesis (4%), and signal transduction (1%). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 18 representative genes demonstrated that about two thirds of the genes displayed different expression profiles between the transcriptional and translational stages. (C) 2012 Elsevier Ltd. All rights reserved.