Journal
JOURNAL OF CEREAL SCIENCE
Volume 50, Issue 2, Pages 266-271Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jcs.2009.06.006
Keywords
Ultrasound-assisted extraction; Defatted wheat germ proteins; Reverse micelles; Forward extraction efficiency
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Funding
- Jiangsu Provincial Natural Science Foundation of China [BK2008102]
- Foundation of Institutions of Higher Education of China [20070295034]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0627]
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In this work, an ultrasound-assisted procedure for the extraction of defatted wheat germ proteins by reverse micelles was established. Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction (UAE) parameters (ultrasonic output power, ultrasonic time and pulse mode) for enhancing the forward extraction efficiency of defatted wheat germ proteins by implementing a three-level, three-variable Box-Behnken experimental design. The independent variable with the largest effect on response was X-2 (ultrasonic time), followed by X, (ultrasonic output power) and X-3 (pulse mode). The optimum extraction conditions were found to be ultrasonic output power 363 W, ultrasonic time 24 min and pulse mode 2.4 s on and 2 s off (2.4 s:2 s). Under these conditions, the forward extraction efficiency of defatted wheat germ proteins can increase from 37% to 57%, and the final protein extraction efficiency by reverse micelles can reach 45.6%, making the advantage of reverse micelles much more obvious than alkaline extraction and isoelectric precipitation, on the separation of protein. (C) 2009 Elsevier Ltd. All rights reserved.
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