Journal
JOURNAL OF CEREAL SCIENCE
Volume 48, Issue 3, Pages 722-728Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jcs.2008.03.007
Keywords
Wheat; Grain hardness; Puroindolines; Grain development; Genetic mapping
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Funding
- Biotechnology and Biological Sciences Research Council (BBSRC) of the UK
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00004953] Funding Source: researchfish
- BBSRC [BBS/E/C/00004953] Funding Source: UKRI
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Transcripts encoding three novel variant forms of puroindoline b have been identified in developing seeds of wheat. These show 57-60% sequence identity with the wild type form of Pin b but all lack one of the three tryptophan residues present in the tryptophan loop region of the wild type protein. Counts of ESTs and array analysis indicate that the transcripts encoding variant forms of Pin b are about an order of magnitude less abundant than those encoding wild type Pin b while array analysis also shows that expression of the variant form 1 declines more rapidly than that of the wild type form during the later stages of grain development. The gene(s) encoding variant form 1, named Pinb-A2, were mapped to the long arm of chromosome 7A of bread wheat where they show linkage to novel QTLs for hardness which have been identified in two doubled haploid populations derived from crosses between hard parental cultivars (Shamrock x Shango, Malacca x Charger). (C) 2008 Elsevier Ltd. All rights reserved.
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